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KMID : 0364820230590030230
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Korean Journal of Microbiology
2023 Volume.59 No. 3 p.230 ~ p.233
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Complete genome sequence of a novel soybean mosaic virus isolated from soybean seeds in Korea
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Shin You-Seop
Park Tae-Seon
Park Ji-Soo
Lee Sa-el
Kim Ye-Yeong
Bak Sang-Min
Lee Su-Heon
Kim Sang-Min
Lee Bong-Chun
Cho Won-Kyong
Hong Jin-Sung
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Abstract
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Soybean mosaic virus (SMV), a member of the genus Potyvirus, primarily damages soybeans. Although most SMV strains do not infect Solanaceae plants, an SMV isolate obtained from soybean seeds (SMV-Gm) was confirmed to induce systemic infection in Nicotiana benthamiana. Its complete genome sequence was determined using reverse transcription (RT)-PCR and cloning, showing that it comprises 9588 nucleotides, with a GC content of 41.65% and a single large polyprotein, consistent with the typical SMV composition.
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KEYWORD
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SMV, complete genome, Nicotiana benthamiana, seed transmission
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